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MATERIALS & METHOD
Ten healthy male subjects of the age range of 28-59 years were selected out of the volunteers who responded to our initial contact. A subject was chosen on the following criteria: (i) pranayamic experience of sufficiently long duration, preferably for some years; (ii) normal health appropriate to age and having not suffered from any major illness; and (iii) willingness to attend a large number of test sessions without dropping out. A full explanation about the study was given to each subject and informed consent obtained. The peak flow rates of the subjects were in the normal range (for Indians) of 390-510 1/min, mean and SD being 451 + 421/ min (determined with Wright’s mini peak flow meter).

All subjects had been practising the Ujjayi pranayama with different ratios of the phase durations of the respiratory cycle. In the typical Ujjayi pranayama 3,4 the respiratory rate is markedly reduced volitionally and each cycle includes successively (i) the phase of inhalation while the glottis is kept partially closed; (ii) the phase of Kumbhak or holding of breath (retention of the inhaled air) during which the glottis is fully closed, and (iii) the phase of a steady exhalation. There may also be a phase of end-expiration Kumbhak or breath-holding in exhaled state, before beginning the next cycle. The cycles were repeated regularly while keeping respective durations of the phases similar in all the cycles of the pranayamic breathing. In typical ratio, the expiration phase is twice that of inspiration while the duration of Kumbhak in end of inspiration is twice that of expiration phase, i.e., 1:4:2 for inspiration: hold: expiration. However, different types of Pranayama differ widely in the ratios of the phase durations. The Kumbhak is to be done in ease and not to be prolonged as in breath-holding for breaking point test. During pranayama, the subject is required to become aware of sensations of passage of air through nasal passages and of filling up of lungs. The entire process of breathing is to be executed without stress and strain, under conscious control, maintaining the ratio of phase durations of each cycle of respiration, carrying on rhythmically, steadily and without jerking movements. In the end-inspiration Kumbhak phase, the perineal muscles are held in contraction, and chin lowered on to the manubrium of sternum (mulabandha, and jalandara bandha). In the end- expiration Kumbhak phase, the abdomen is drawn inwards and backwards with diaphragm in raised position (uddiyan bandha).

Since the duration of the inspiratory Kumbhak is known to be an all-important variable of the Ujjayi Pranayama, we have categorised the ten subjects into two groups of five each, viz., long and short Kumbhak types. In the long Kumbhak group (LK), the Kumbhak (in inspiration) phase duration was 50.4±6.6 per cent of the total duration of respiratory cycle. In the short Kumbhak group (SK) the Kumbhak phase was only about 22.2 ± 10.6 percent of the total cycle duration. The inspiration-Kumbhak-expiration duration’s ratio was about 1:4:2 in the long Kumbhak group, and about 1:1 (or less):1 in the short, Kumbhak group. Three subjects in each group also had the end- expiration Kumbhak. However, there were no differences in other respects, i.e., in the base-line values of the two groups of subjects. Their average ages were not significantly different (Student’s ‘t’ test), the values being 45.2 ± 7.4 years for the SK group and 38.4 ± 13.9 years for the LK group. Besides, the pre-pranayamic base-line values of pulmonary ventilation were not different, the value of the SK group was 15.0±5.8 1/min BTPS and of the LK group was 10.3 ± 2.5 l/min. BTPS (Table 1). The base-line oxygen consumption also, of the two groups was not different (Student’s ‘t’ test), the average values being 269.6 ± 127 ml/min STPD for the SK group and 320.8±96.7 ml/min STPD for the LK group (Table II).

The oxygen consumption was recorded using the closed circuit method of the Benedict-Roth apparatus5. In this method the subject breathes into an oxygen tank, the exhaled carbon dioxide having been excluded from entering the tank by absorbing it in soda lime.
 
Table I
Pulmonary ventilation
(1 /min BTPS) (Data are mean ± SD)
 
Short Kumbhak pranayama Long Kumbhak pranayama
Subjects Repli-cation Before Pranayama During Prana-yama After Prana-yama Subj-ects Repli-cation Before Prana - yama During Prana - yama After Prana - yama
STY 14 15.8 ± 2.8 7.4 ± 1.9 12.8 ± 2.0 NA 13 6.6 ± 2.2 4.5 ± 1.0 6.2 ± 1.4
VIS 10 15.0 ± 3.9 6.9 ± 1.0 14.5 ± 2.6 RJ 12 9.5 ± 2.5 6.2 ± 1.2 10.8 ± 2.5
NAR 12 7.7 ± 2.8 5.3 ± 2.3 7.2 ± 2.3 SW 15 13.7 ± 4.9 4.0 ± 0.8 13.6 ± 4.2
VJ 10 12.9 ± 5.4 8.2 ± 1.1 16.9 ± 6.6 STH 11 11.0 ± 4.3 6.0 ± 0.8 11.2 ± 2.9
AI 7 23.7 ± 6.7 8.3 ± 2.0 22.9 ± 7.3 SN 10 10.7 ± 6.7 2.5 ± 0.7 11.9 ± 2.9
 
Paired ‘t’ test against ‘before’ P< 0.025 Not significant 10.3 ± 2.8 P < 0.025 Not Significant
Mean ± SD   15.0 ± 5.8 7.2 ± 1.2 14.8 ± 5.7     10.3 ± 2.5 1.6 ± 1.5 10.7 ± 2.7
% of ‘bfore’   (100.0%) (48.0%) (98.6%)     (100.0%) (46.6%) (103.8%)
 
 
Difference between ‘before’ values of the two pranayama types was not significant (Student’s ‘t’ teset; P>0.1)
 
Table II
Oxygen consumption
(ml/min STPD) Data are mean ± SD)
 
Short Kumbhak pranayama Long Kumbhak pranayama
Subjects Repli-cation Before Pranayama During Prana-yama After Prana-yama Subj-ects Repli-cation Before Prana - yama During Prana - yama After Prana - yama
STY 14 255 ± 85.0 405 ± 273.3 330 ± 171.6 NA 13 179 ± 65.2 104 ± 40.3 131 ± 63.8
VIS 10 321 ± 87.9 390 ± 129.2 238 ± 119.0 RJ 12 286 ± 138.7 190 ± 71.9 205 ± 84.9
NAR 12 442 ± 154.9 715 ± 286.7 379 ± 101.0 SW 15 432 ± 224.6 342 ± 132.8 355 ± 121.0
VJ 10 236 ± 67.4 339 ± 58.0 268 ± 179.9 STH 11 325 ± 176.9 310 ± 149.7 373 ± 164.1
AI 7 94 ± 53.4 205 ± 169.0 118 ± 75.0 SN 10 382 ± 100.0 349 ± 84.2 320 ± 120.0
 
Paired ‘t’ test P< 0.05 Not significant 10.3 ± 2.8 P < 0.025 Not Significant
Mean ± SD   269.6 ± 127 4.10 ± 187.4 266.6 ± 99.4     320 ± 96.7 259.0 ± 107.6 276.8 ± 104.4
% of ‘bfore’   (100.0%) (152%) (98.8%)     (100.0%) (81%) (86%)
 
 
Difference between ‘before’ values of the two pranayama types was not significant (Student ‘t” test; P>0.4)
 
The difference between the initial and final volumes of oxygen in the tank is the oxygen consumed by the subject in a given period of breathing. In normal tidal type of breathing, the end-expiratory points can be joined into a slanted straight line, and the difference between the two ends of the line gives the value of oxygen consumed. In the present study involving a specialized type of pranayama breathing also, the same method is useful if the line is drawn as a best-fitting average connecting the end-expiratory points. The accuracy of this value compared well (90%) against a value obtained by summing the oxygen values calculated separately for individual breaths during the period. When the amplitudes of breaths varied much more, that session was not included in the analysis. Moreover, the value for each individual being the mean of data of several test sessions, even the small errors of individual sessions would get evened out in this study. In each experimental (test) session, oxygen consumption assessments were made for the three periods:

The pre or before, the during, the post or after pranayamic practice, as illustrated in Figs 1 and 2. Each of these test periods was of about 4 min duration. The recording laboratory was air conditioned to about 270C with relative humidity of 50 per cent. Since the subjects had to wear the face mask and breathe through the valves of the closed circuit apparatus they had not used the nostril manipulations and full chin-lock position. In between the three periods (before, during, and after) a pause or an interruption for 30 sec with breathing air was allowed while replenishing oxygen in the spirometer bell and readjusting the writing pen level. The spirometer record provided the values of tidal volume, respiratory rate and also the oxygen consumption of each of the periods. From these, the volume of gas ventilated was calculated. The oxygen values having been obtained under room temperature and pressure, they were corrected for standard temperature and pressure. Whether a change occurred in oxygen consumed during the test period of pranayama and in the period after pranayama, was assessed by comparing with the control value obtained in
 
   
Fig. 1. Representative record made on a polygraph with mercury strain guage connected to the chest, and subject parallely connected to the Benedict-Roth spirometer while, (A) breathing air with face mask valve opened to outside air, (B) breathing oxygen through the closed circuit system of the spirometer during pre-pranayamic control period, and (C) during the pranayamic practice period (inspiration recorded upwards), (D) records made on the Benedict-Roth spirometer (expiration is upwards), before, during, and after the pranayama, this case being of short kumbhak type and having both inspiration and expiratory kumbhak phases. Fig. 2. Examples of short and long kumbhak types of records of pranayamas on Benedict-Roth spirometer. Pranayama lower two subjects have both inspiratory and expiratory kumbhak phases, SB of short type, and NG of long type. (top figure) practices long type with only the inspiratory. Kumbhak phase.
 
The period preceding (before) the pranayama of the same experimental session, so that the status of diet or metabolism and other unknown variables prevailing on that day and time of recording would be common for both control and test data thereby not affecting the inferences. On each subject several test sessions were repeated and the average of these repeated measurements was considered as the value for that subject. Such averaged values of the three test conditions of the five subjects of the group were averaged and treated statistically 6-8. Statistical significance of difference of oxygen consumption of the pranayamic breathing, or of post-pranayamic period, from the pre-pranayamic value was assessed by the matched pairs ‘t’ test7.8. Comparisons across groups were made by Student’s ‘t’ test. The significance of effects of the three treatment conditions (before, during, after) for the five subjects of the group were also assessed by the method of analysis of variance 6 i.e., two-way ANOVA.
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